Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases

Buckenmaier, Stephan M.C.; McCalley, David V.; Euerby, Melvin R.

doi:10.1021/ac0202381

All Outputs (4)

Overload for ionized solutes in reversed-phase high-performance liquid chromatography (2006)
Journal Article

Overloading occurs for submicrogram quantities of ionized solutes particularly when using low ionic strength mobile phases at low pH (e.g., formic acid), even with highly inert silica RP-HPLC columns of normal dimensions. Much higher loads can produc... Read More about Overload for ionized solutes in reversed-phase high-performance liquid chromatography.

Effect of buffer on peak shape of peptides in reversed-phase high performance liquid chromatography (2004)
Journal Article

The effect of changing the buffer at constant low pH in the mobile phase is investigated with respect to the separation of a mixture of basic peptides. Considerably worse peak shapes, leading to poorer resolution of complex peptide mixtures, were obt... Read More about Effect of buffer on peak shape of peptides in reversed-phase high performance liquid chromatography.

Rationalization of retention and overloading behavior of basic compounds in reversed-phase HPLC using low ionic strength buffers suitable for mass spectrometric detection (2003)
Journal Article

The retention and overloading behavior of some basic (and acidic) compounds has been studied on different RP-HPLC columns in buffers of varying ionic strength. Anomalous retention patterns of acids and bases were found on one phase in low-pH, volatil... Read More about Rationalization of retention and overloading behavior of basic compounds in reversed-phase HPLC using low ionic strength buffers suitable for mass spectrometric detection.

Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases (2002)
Journal Article

The separation of ionized bases by reversed-phase liquid chromatography with alkyl silica columns often leads to severely tailed bands that are highly detrimental. Band shape and its dependence on sample mass are notably different when mobile-phase p... Read More about Overloading study of bases using polymeric RP-HPLC columns as an aid to rationalization of overloading on silica-ODS phases.