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Managing the column equilibration time in hydrophilic interaction chromatography (2019)
Journal Article
McCalley, D. V. (2020). Managing the column equilibration time in hydrophilic interaction chromatography. Journal of Chromatography A, 1612, https://doi.org/10.1016/j.chroma.2019.460655

For a wide variety of hydrophilic interaction chromatography stationary phases, a repeatable partial equilibration was demonstrated in gradient elution after purging with as little as 12 column volumes of mobile phase. Relative standard deviations of... Read More about Managing the column equilibration time in hydrophilic interaction chromatography.

Evaluation of additives on reversed-phase chromatography of monoclonal antibodies using a 1000 Å stationary phase (2019)
Journal Article
McCalley, D. V., & Guillarme, D. (2020). Evaluation of additives on reversed-phase chromatography of monoclonal antibodies using a 1000 Å stationary phase. Journal of Chromatography A, 1610, https://doi.org/10.1016/j.chroma.2019.460562

© 2019 Elsevier B.V. A wide pore (1000 Å) diphenyl stationary phase was evaluated for the analysis of monoclonal antibodies (mAbs), comparing a conventional mobile phase of acetonitrile-water containing overall 0.1% trifluoracetic acid (TFA) with a s... Read More about Evaluation of additives on reversed-phase chromatography of monoclonal antibodies using a 1000 Å stationary phase.

Hydrophilic interaction liquid chromatography: An update (2019)
Journal Article
McCalley, D. V. (2019). Hydrophilic interaction liquid chromatography: An update. Lc Gc Europe, 32(3), 114-125

This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) and covers recent ideas on the mechanism of separation, and how it may be manipulated to suit the separation of particular sample types. The advantage... Read More about Hydrophilic interaction liquid chromatography: An update.