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A systematic investigation of the effect of sample diluent on peak shape in hydrophilic interaction liquid chromatography (2010)
Journal Article
Ruta, J., Rudaz, S., McCalley, D. V., Veuthey, J. L., & Guillarme, D. (2010). A systematic investigation of the effect of sample diluent on peak shape in hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1217(52), 8230-8240. https://doi.org/10.1016/j.chroma.2010.10.106

The aim of this study was to evaluate the importance of sample diluents to improve peak shapes in hydrophilic interaction liquid chromatography (HILIC), using low molecular weight (

Instrumental considerations for the effective operation of short, highly efficient fused-core columns. Investigation of performance at high flow rates and elevated temperatures (2010)
Journal Article
McCalley, D. V. (2010). Instrumental considerations for the effective operation of short, highly efficient fused-core columns. Investigation of performance at high flow rates and elevated temperatures. Journal of Chromatography A, 1217(27), 4561-4567. https://doi.org/10.1016/j.chroma.2010.04.070

Fused core or superficially porous columns offer the advantages of higher efficiency compared with totally porous columns of the same particle size, but similar operating pressures. However, their performance may be adversely affected by extra-column... Read More about Instrumental considerations for the effective operation of short, highly efficient fused-core columns. Investigation of performance at high flow rates and elevated temperatures.

Study of the selectivity, retention mechanisms and performance of alternative silica-based stationary phases for separation of ionised solutes in hydrophilic interaction chromatography (2010)
Journal Article
McCalley, D. V. (2010). Study of the selectivity, retention mechanisms and performance of alternative silica-based stationary phases for separation of ionised solutes in hydrophilic interaction chromatography. Journal of Chromatography A, 1217(20), 3408-3417. https://doi.org/10.1016/j.chroma.2010.03.011

The separation of a mixture of neutral, strongly acidic and strongly basic compounds was studied in hydrophilic interaction chromatography using a bare silica phase, and bonded silica phases with diol, zwitterionic, amide and hydrophilic/hydrophobic... Read More about Study of the selectivity, retention mechanisms and performance of alternative silica-based stationary phases for separation of ionised solutes in hydrophilic interaction chromatography.

The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations (2010)
Journal Article
McCalley, D. V. (2010). The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations. Journal of Chromatography A, 1217(6), 858-880. https://doi.org/10.1016/j.chroma.2009.11.068

This review considers some of the difficulties encountered with the analysis of ionised bases using reversed-phase chromatography, such as detrimental interaction with column silanol groups, and overloading which both lead to poor peak shapes. Method... Read More about The challenges of the analysis of basic compounds by high performance liquid chromatography: Some possible approaches for improved separations.

Further investigations of the effect of pressure on retention in ultra-high-pressure liquid chromatography (2010)
Journal Article
Fallas, M. M., Neue, U. D., Hadley, M. R., & McCalley, D. V. (2010). Further investigations of the effect of pressure on retention in ultra-high-pressure liquid chromatography. Journal of Chromatography A, 1217(3), 276-284. https://doi.org/10.1016/j.chroma.2009.11.041

In this study, we investigated further the large increases in retention with pressure that we observed previously in RP-LC especially for ionised solutes. These findings were initially confirmed on a conventional silica C18 column, which gave extreme... Read More about Further investigations of the effect of pressure on retention in ultra-high-pressure liquid chromatography.