All Outputs (2)

Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH (2006)
Journal Article
Davies, N. H., Euerby, M. R., & McCalley, D. V. (2006). Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH. Journal of Chromatography A, 1119(1-2), 11-19. https://doi.org/10.1016/j.chroma.2005.11.096

The retention and overloading properties for eight basic solutes and two quaternary ammonium compounds were studied over the pH range 2.7-10.0 using phosphate and carbonate buffers. At low pH, a hybrid inorganic-organic silica-ODS phase (XTerra RP-18... Read More about Study of overload for basic compounds in reversed-phase high performance liquid chromatography as a function of mobile phase pH.

Overload for ionized solutes in reversed-phase high-performance liquid chromatography (2006)
Journal Article
McCalley, D. V. (2006). Overload for ionized solutes in reversed-phase high-performance liquid chromatography. Analytical Chemistry, 78(8), 2532-2538. https://doi.org/10.1021/ac052098b

Overloading occurs for submicrogram quantities of ionized solutes particularly when using low ionic strength mobile phases at low pH (e.g., formic acid), even with highly inert silica RP-HPLC columns of normal dimensions. Much higher loads can produc... Read More about Overload for ionized solutes in reversed-phase high-performance liquid chromatography.