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Managing the column equilibration time in hydrophilic interaction chromatography (2019)
Journal Article
McCalley, D. V. (2020). Managing the column equilibration time in hydrophilic interaction chromatography. Journal of Chromatography A, 1612, https://doi.org/10.1016/j.chroma.2019.460655

For a wide variety of hydrophilic interaction chromatography stationary phases, a repeatable partial equilibration was demonstrated in gradient elution after purging with as little as 12 column volumes of mobile phase. Relative standard deviations of... Read More about Managing the column equilibration time in hydrophilic interaction chromatography.

Hydrophilic interaction liquid chromatography: An update (2019)
Journal Article
McCalley, D. V. (2019). Hydrophilic interaction liquid chromatography: An update. Lc Gc Europe, 32(3), 114-125

This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) and covers recent ideas on the mechanism of separation, and how it may be manipulated to suit the separation of particular sample types. The advantage... Read More about Hydrophilic interaction liquid chromatography: An update.

Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration (2018)
Journal Article
Heaton, J. C., Smith, N. W., & McCalley, D. V. (2019). Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration. Analytica Chimica Acta, 1045, 141-151. https://doi.org/10.1016/j.aca.2018.08.051

© 2018 Elsevier B.V. The separation of some zwitterionic, basic and neutral antibiotic and antiretroviral compounds was studied using hydrophilic interaction chromatography (HILIC) on bare silica, bonded amide and urea superficially porous phases. T... Read More about Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration.

A study of column equilibration time in hydrophilic interaction chromatography (2018)
Journal Article
McCalley, D. V. (2018). A study of column equilibration time in hydrophilic interaction chromatography. Journal of Chromatography A, 1554, 61-70. https://doi.org/10.1016/j.chroma.2018.04.016

© 2018 Elsevier B.V. The time taken to achieve full column equilibration for isocratic analysis of acidic, basic, and neutral solutes in hydrophilic interaction liquid chromatography (HILIC) was compared using the mobile phase disturbance method, col... Read More about A study of column equilibration time in hydrophilic interaction chromatography.

A study of the analysis of acidic solutes by hydrophilic interaction chromatography (2017)
Journal Article
McCalley, D. V. (2018). A study of the analysis of acidic solutes by hydrophilic interaction chromatography. Journal of Chromatography A, 1534, 64-74. https://doi.org/10.1016/j.chroma.2017.12.045

© 2017 Elsevier B.V. The analysis of acidic solutes was compared on a cross-linked bonded amino phase and a neutral hybrid inorganic-organic amide phase, previously shown to give reasonable retention of acidic solutes. The amino column gave strong se... Read More about A study of the analysis of acidic solutes by hydrophilic interaction chromatography.

Understanding and manipulating the separation in hydrophilic interaction liquid chromatography (2017)
Journal Article
McCalley, D. V. (2017). Understanding and manipulating the separation in hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1523, 49-71. https://doi.org/10.1016/j.chroma.2017.06.026

© 2017 Elsevier B.V. Hydrophilic interaction liquid chromatography (HILIC) has emerged as a valuable complimentary technique to reversed-phase (RP), being especially suited for the analysis of polar and ionised solutes, which are difficult to retain... Read More about Understanding and manipulating the separation in hydrophilic interaction liquid chromatography.

Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography (2016)
Journal Article
McCalley, D. V. (2017). Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography. Journal of Chromatography A, 1483, 71-79. https://doi.org/10.1016/j.chroma.2016.12.035

© 2016 Elsevier B.V. Trifluoracetic acid (TFA) added to the aqueous acetonitrile mobile phase induces some unexpected changes in the ionic component of retention in hydrophilic interaction separations when using Type B silica and amide-bonded silica... Read More about Effect of mobile phase additives on solute retention at low aqueous pH in hydrophilic interaction liquid chromatography.

Recent developments in support materials for use in high performance liquid chromatography (2016)
Journal Article
McCalley, D. V. (2016). Recent developments in support materials for use in high performance liquid chromatography

A number of advances have taken place in the last decade in the area of support materials for high performance liquid chromatography (HPLC). These developments have included the use of very small porous particles (< 2.0 micron diameter), new types of... Read More about Recent developments in support materials for use in high performance liquid chromatography.

Distinction of synthetic dl-α-tocopherol from natural vitamin E (d-α-tocopherol) by reversed-phase liquid chromatography. Enhanced selectivity of a polymeric C18 stationary phase at low temperature and/or at high pressure (2016)
Journal Article
Yui, Y., Miyazaki, S., Ma, Y., Ohira, M., Fiehn, O., Ikegami, T., …Tanaka, N. (2016). Distinction of synthetic dl-α-tocopherol from natural vitamin E (d-α-tocopherol) by reversed-phase liquid chromatography. Enhanced selectivity of a polymeric C18 stationary phase at low temperature and/or at high pressure. Journal of Chromatography A, 1450, 45-52. https://doi.org/10.1016/j.chroma.2016.04.076

© 2016 Elsevier B.V. Separation of diastereomers of DL-α-tocopherol was studied by reversed-phase liquid chromatography using three types of stationary phases, polymeric ODS, polymeric C30, and monomeric ODS. Polymeric ODS stationary phase (Inertsil... Read More about Distinction of synthetic dl-α-tocopherol from natural vitamin E (d-α-tocopherol) by reversed-phase liquid chromatography. Enhanced selectivity of a polymeric C18 stationary phase at low temperature and/or at high pressure.

Core-Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography (2016)
Journal Article
Tanaka, N., & McCalley, D. V. (2016). Core-Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography. Analytical Chemistry, 88(1), 279-298. https://doi.org/10.1021/acs.analchem.5b04093

A review of the developments that have taken place in the area of supports for high performance liquid chromatography in recent years.

Some factors that can lead to poor peak shape in hydrophilic interaction chromatography, and possibilities for their remediation (2016)
Journal Article
Heaton, J. C., Heaton, J., & McCalley, D. V. (2016). Some factors that can lead to poor peak shape in hydrophilic interaction chromatography, and possibilities for their remediation. Journal of Chromatography A, 1427, 37-44. https://doi.org/10.1016/j.chroma.2015.10.056

© 2015 Elsevier B.V. Some factors which present difficulties for obtaining good peak shape in hydrophilic interaction chromatography (HILIC) were studied. The effect of injection solvent composition and volume was systematically investigated using a... Read More about Some factors that can lead to poor peak shape in hydrophilic interaction chromatography, and possibilities for their remediation.

Performance of charged aerosol detection with hydrophilic interaction chromatography (2015)
Journal Article
Russell, J. J., Heaton, J. C., Underwood, T., Boughtflower, R., & McCalley, D. V. (2015). Performance of charged aerosol detection with hydrophilic interaction chromatography. Journal of Chromatography A, 1405, 72-84. https://doi.org/10.1016/j.chroma.2015.05.050

© 2015 The Authors. The performance of the charged aerosol detector (CAD) was investigated using a diverse set of 29 solutes, including acids, bases and neutrals, over a range of mobile phase compositions, particularly with regard to its suitability... Read More about Performance of charged aerosol detection with hydrophilic interaction chromatography.

The impact of pressure and frictional heating on retention, selectivity and efficiency in ultra-high-pressure liquid chromatography (2014)
Journal Article
McCalley, D. V. (2014). The impact of pressure and frictional heating on retention, selectivity and efficiency in ultra-high-pressure liquid chromatography. Trends in Analytical Chemistry, 63, 31-43. https://doi.org/10.1016/j.trac.2014.06.024

© 2014 Elsevier B.V. The effects of pressure and frictional heating deserve serious consideration in ultra-high-pressure liquid chromatography (UHPLC) separations, as the pressures used can be three times greater than those in conventional high-perfo... Read More about The impact of pressure and frictional heating on retention, selectivity and efficiency in ultra-high-pressure liquid chromatography.

Effect of pressure on the selectivity of polymeric C18 and C30 stationary phases in reversed-phase liquid chromatography. Increased separation of isomeric fatty acid methyl esters, triacylglycerols, and tocopherols at high pressure (2014)
Journal Article
Okusa, K., Iwasaki, Y., Kuroda, I., Miwa, S., Ohira, M., Nagai, T., …Tanaka, N. (2014). Effect of pressure on the selectivity of polymeric C18 and C30 stationary phases in reversed-phase liquid chromatography. Increased separation of isomeric fatty acid methyl esters, triacylglycerols, and tocopherols at high pressure. Journal of Chromatography A, 1339, 86-95. https://doi.org/10.1016/j.chroma.2014.02.077

A high-density, polymeric C18 stationary phase (Inertsil ODS-P) or a polymeric C30 phase (Inertsil C30) provided improved resolution of the isomeric fatty acids (FAs), FA methyl esters (FAMEs), triacylglycerols (TAGs), and tocopherols with an increas... Read More about Effect of pressure on the selectivity of polymeric C18 and C30 stationary phases in reversed-phase liquid chromatography. Increased separation of isomeric fatty acid methyl esters, triacylglycerols, and tocopherols at high pressure.

Comparison of peak shape in hydrophilic interaction chromatography using acidic salt buffers and simple acid solutions (2014)
Journal Article
Heaton, J. C., Russell, J. J., Underwood, T., Boughtflower, R., & McCalley, D. V. (2014). Comparison of peak shape in hydrophilic interaction chromatography using acidic salt buffers and simple acid solutions. Journal of Chromatography A, 1347, 39-48. https://doi.org/10.1016/j.chroma.2014.04.026

The retention and peak shape of neutral, basic and acidic solutes was studied on hydrophilic interaction chromatography (HILIC) stationary phases that showed both strong and weak ionic retention characteristics, using aqueous-acetonitrile mobile phas... Read More about Comparison of peak shape in hydrophilic interaction chromatography using acidic salt buffers and simple acid solutions.

Comparison of the kinetic performance and retentivity of sub-2μm core-shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography (2014)
Journal Article
Heaton, J. C., & McCalley, D. V. (2014). Comparison of the kinetic performance and retentivity of sub-2μm core-shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography. Journal of Chromatography A, 1371, 106-116. https://doi.org/10.1016/j.chroma.2014.10.013

© 2014 Elsevier B.V. The separation performance and retention properties of four sub-2μm underivatised silica materials were evaluated in the hydrophilic interaction chromatography (HILIC) mode. These included an organic/inorganic hybrid silica, conv... Read More about Comparison of the kinetic performance and retentivity of sub-2μm core-shell, hybrid and conventional bare silica phases in hydrophilic interaction chromatography.

Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography (2013)
Journal Article
Kumar, A., Heaton, J. C., & McCalley, D. V. (2013). Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography. Journal of Chromatography A, 1276, 33-46. https://doi.org/10.1016/j.chroma.2012.12.037

Retention data for a series of 29 compounds comprising acids, bases and neutrals were obtained on six different hydrophilic interaction (HILIC) columns including bare silica, zwitterionic and those bonded with neutral bonded ligands. The principal ai... Read More about Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography.

The effect of pressure and mobile phase velocity on the retention properties of small analytes and large biomolecules in ultra-high pressure liquid chromatography (2012)
Journal Article
Fekete, S., Veuthey, J. L., McCalley, D. V., & Guillarme, D. (2012). The effect of pressure and mobile phase velocity on the retention properties of small analytes and large biomolecules in ultra-high pressure liquid chromatography. Journal of Chromatography A, 1270, 127-138. https://doi.org/10.1016/j.chroma.2012.10.056

A possible complication of ultra-high pressure liquid chromatography (UHPLC) is related to the effect of pressure and mobile phase velocity on the retention properties of the analytes. In the present work, numerous model compounds have been selected... Read More about The effect of pressure and mobile phase velocity on the retention properties of small analytes and large biomolecules in ultra-high pressure liquid chromatography.

A comparison of overload behaviour for some sub 2μm totally porous and sub 3μm shell particle columns with ionised solutes (2012)
Journal Article
Buckenmaier, S. M. C., Fallas, M. M., Buckenmaier, S. M., & McCalley, D. V. (2012). A comparison of overload behaviour for some sub 2μm totally porous and sub 3μm shell particle columns with ionised solutes. Journal of Chromatography A, 1235, 49-59. https://doi.org/10.1016/j.chroma.2012.02.027

The overloading performance of some 2.7 μm shell and sub 2 μm totally porous columns, including one pair manufactured from similar materials with similar bonding chemistries, was compared using strongly acidic and basic probe compounds. In general, t... Read More about A comparison of overload behaviour for some sub 2μm totally porous and sub 3μm shell particle columns with ionised solutes.