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Determining the sensitivity of a cell to a drug

Salisbury, Vyv; Alloush, H; Smith, A

Authors

Vyv Salisbury

H Alloush

A Smith



Abstract

The present invention provides an in vitro method for determining the resistance or sensitivity of a cell line or patient sample to a deoxyribonucleoside kinase-dependent drug, wherein the method comprises the steps of: (i) treating a patient sample or cell line, or a portion thereof, with a deoxyribonucleoside kinase-dependent drug; (ii) lysing the cells of the patient sample or cell line from step (i); (iii) optionally, mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for deoxyribonucleoside kinase; (iv) mixing a portion of the cell lysate from (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase and a deoxyribonucleoside kinase transcription promoter; (v) mixing a portion of the cell lysate from step (ii) with a bioluminescent reporter bacteria incorporating a gene coding for a deoxyribonucleoside kinase, a deoxyribonucleoside kinase transcription promoter and a dephosphorylating agent; and (vi) measuring the bioluminescence of each of the mixtures from steps (iii) to (v), wherein the comparative levels of bioluminescence of each of the mixtures provides a measure of the resistance or sensitivity to the drug.

Citation

Salisbury, V., Alloush, H., & Smith, A. (2010). Determining the sensitivity of a cell to a drug. WO/2010/015844

Publication Date Feb 11, 2010
Deposit Date Dec 20, 2010
Publicly Available Date Mar 29, 2024
Keywords drug sensitivity, bioluminescece
Public URL https://uwe-repository.worktribe.com/output/981315
Publisher URL http://www.wipo.int/patentscope/search/en/detail.jsf?docId=WO2010015844
Additional Information Patent Applicant : Randox Laboratories and University of the West of England


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