Magnetizable antibody-like proteins

Dehal, Prabhjyot Kaur; Livingston, Christine Frances; Dunn, Claire Geekie; Buick, Richard; Luxton, Richard W.; Pritchard, David John

Abstract

Use of paramagnetic particles to isolate molecules or cells from complex media is well established. Typically, particles are manufactured and coated with a biological molecule that confers specific biorecognition. Incubation of particles with sample and exposure to magnetic fields isolates the species of interest. We have designed, produced and assessed magnetized fusion proteins consisting of the antigen-binding portion of an antibody (single chain variable fraction; scFv) fused to the heavy chain of the iron-binding protein ferritin. The fusion protein subunits expressed in E. coli assemble to form a fusion protein consisting of a ferritin sphere with scFvs on the surface. The fusion proteins were chemically magnetized by introducing a paramagnetic iron core. The resultant fusion protein was shown to be magnetizable and capable of binding target antigens. These "organic" magnetizable particles possess a number of theoretical advantages over traditional inorganic particles. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

Citation

Dehal, P. K., Livingston, C. F., Dunn, C. G., Buick, R., Luxton, R. W., & Pritchard, D. J. (2010). Magnetizable antibody-like proteins. Biotechnology Journal, 5(6), 596-604. https://doi.org/10.1002/biot.200900273

• Journal Article Type: Article
• Publication Date: Jun 1, 2010
• Deposit Date: Jan 13, 2011
• Journal: Biotechnology Journal
• Print ISSN: 1860-6768
• Electronic ISSN: 1860-7314
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 6
• Pages: 596-604
• DOI: https://doi.org/10.1002/biot.200900273
• Keywords: analytical biotechnology, antibody, ferritin, paramagnetic, scFv
• Public URL: https://uwe-repository.worktribe.com/output/978465
• Publisher URL: http://dx.doi.org/10.1002/biot.200900273