Abhinav Kumar
Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography
Kumar, Abhinav; Heaton, James C.; McCalley, David V.
Authors
James C. Heaton
Professor David McCalley David.Mccalley@uwe.ac.uk
Professor in Bio-Analytical Science
Abstract
Retention data for a series of 29 compounds comprising acids, bases and neutrals were obtained on six different hydrophilic interaction (HILIC) columns including bare silica, zwitterionic and those bonded with neutral bonded ligands. The principal aim of the work was to evaluate the effect of various experimental variables such as the nature of the stationary phase, buffer pH, buffer concentration, organic solvent and its concentration, and temperature, in order to determine which factors gave the most effect on the selectivity of the separation. The influence of solute properties on the separation, such as log. D values, was also considered. In this way, it was hoped to provide a practical guide to aid in the selection of conditions to achieve a separation in HILIC. The nature of the stationary phase was found to have the greatest effect on selectivity. © 2012 Elsevier B.V.
Citation
Kumar, A., Heaton, J. C., & McCalley, D. V. (2013). Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography. Journal of Chromatography A, 1276, 33-46. https://doi.org/10.1016/j.chroma.2012.12.037
Journal Article Type | Article |
---|---|
Publication Date | Feb 8, 2013 |
Journal | Journal of Chromatography A |
Print ISSN | 0021-9673 |
Electronic ISSN | 1873-3778 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 1276 |
Pages | 33-46 |
DOI | https://doi.org/10.1016/j.chroma.2012.12.037 |
Keywords | HPLC, hydrophilic interaction, chromatography, HILIC, selectivity |
Public URL | https://uwe-repository.worktribe.com/output/934880 |
Publisher URL | http://dx.doi.org/10.1016/j.chroma.2012.12.037 |
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