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Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography

Kumar, Abhinav; Heaton, James C.; McCalley, David V.

Authors

Abhinav Kumar

James C. Heaton



Abstract

Retention data for a series of 29 compounds comprising acids, bases and neutrals were obtained on six different hydrophilic interaction (HILIC) columns including bare silica, zwitterionic and those bonded with neutral bonded ligands. The principal aim of the work was to evaluate the effect of various experimental variables such as the nature of the stationary phase, buffer pH, buffer concentration, organic solvent and its concentration, and temperature, in order to determine which factors gave the most effect on the selectivity of the separation. The influence of solute properties on the separation, such as log. D values, was also considered. In this way, it was hoped to provide a practical guide to aid in the selection of conditions to achieve a separation in HILIC. The nature of the stationary phase was found to have the greatest effect on selectivity. © 2012 Elsevier B.V.

Citation

Kumar, A., Heaton, J. C., & McCalley, D. V. (2013). Practical investigation of the factors that affect the selectivity in hydrophilic interaction chromatography. Journal of Chromatography A, 1276, 33-46. https://doi.org/10.1016/j.chroma.2012.12.037

Journal Article Type Article
Publication Date Feb 8, 2013
Journal Journal of Chromatography A
Print ISSN 0021-9673
Electronic ISSN 1873-3778
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 1276
Pages 33-46
DOI https://doi.org/10.1016/j.chroma.2012.12.037
Keywords HPLC, hydrophilic interaction, chromatography, HILIC, selectivity
Public URL https://uwe-repository.worktribe.com/output/934880
Publisher URL http://dx.doi.org/10.1016/j.chroma.2012.12.037