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An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome

de Costello, Ben Lacy; Probert, Chris S.; Ahmed, Iftikhar; de Lacy Costello, Ben; Greenwood, Rosemary; Probert, C.S.J.; Ratcliffe, Norman M.

Authors

Ben Lacy de Costello

Chris S. Probert

Iftikhar Ahmed

Rosemary Greenwood

C.S.J. Probert

Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science



Abstract

Diagnosing irritable bowel syndrome (IBS) can be a challenge; many clinicians resort to invasive investigations in order to rule out other diseases and reassure their patients. Volatile organic metabolites (VOMs) are emitted from feces; understanding changes in the patterns of these VOMs could aid our understanding of the etiology of the disease and the development of biomarkers, which can assist in the diagnosis of IBS. We report the first comprehensive study of the fecal VOMs patterns in patients with diarrhea-predominant IBS (IBS-D), active Crohn's disease (CD), ulcerative colitis (UC) and healthy controls. 30 patients with IBS-D, 62 with CD, 48 with UC and 109 healthy controls were studied. Diagnosis of IBS-D was made using the Manning criteria and all patients with CD and UC met endoscopic, histologic and/or radiologic criteria. Fecal VOMs were extracted by solid phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). 240 VOMs were identified. Univariate analysis showed that esters of short chain fatty acids, cyclohexanecarboxylic acid and its ester derivatives were associated with IBS-D (p

Citation

Probert, C. S., de Costello, B. L., Ahmed, I., Greenwood, R., de Lacy Costello, B., Ratcliffe, N. M., & Probert, C. (2013). An Investigation of Fecal Volatile Organic Metabolites in Irritable Bowel Syndrome. PLoS ONE, 8(3), https://doi.org/10.1371/journal.pone.0058204

Journal Article Type Article
Publication Date Mar 13, 2013
Journal PLoS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 8
Issue 3
DOI https://doi.org/10.1371/journal.pone.0058204
Keywords irritable bowel syndrome, fecal volatile organic metabolites
Public URL https://uwe-repository.worktribe.com/output/933741
Publisher URL http://www.plosone.org/