The dual role of paramagnetic particles for integrated lysis and measurement in a rapid immunoassay for intracellular proteins

Sharif, Elham; Luxton, Richard; Syarif, Elham; Kiely, Janice; Luxton, Richard W.; Wraith, Patrick

Abstract

A novel, integrated lysis and immunoassay methodology and system for intracellular protein measurement are described. The method uses paramagnetic particles both as a lysis agent and assay label resulting in a rapid test requiring minimal operator intervention, the test being homogeneous and completed in less than 10 min. A design study highlights the critical features of the magnetic detection system used to quantify the paramagnetic particles and a novel frequency-locked loop-based magnetometer is presented. A study of paramagnetic particle enhanced lysis demonstrates that the technique is more than twice as efficient at releasing intracellular protein as ultrasonic lysis alone. Results are presented for measurements of intracellular prostate specific antigen in an LNCAP cell line. This model was selected to demonstrate the rapidity and efficiency of intracellular protein quantification. It was shown that, on average, LNCAP cells contained 0.43 fg of prostate specific antigen. This system promises an attractive solution for applications that require a rapid determination of intracellular proteins. © 1964-2012 IEEE.

Citation

Luxton, R., Sharif, E., Syarif, E., Kiely, J., Wraith, P., & Luxton, R. W. (2013). The dual role of paramagnetic particles for integrated lysis and measurement in a rapid immunoassay for intracellular proteins. IEEE Transactions on Biomedical Engineering, 60(5), 1209-1216. https://doi.org/10.1109/TBME.2012.2228642

• Journal Article Type: Article
• Publication Date: May 1, 2013
• Journal: IEEE Transactions on Biomedical Engineering
• Print ISSN: 0018-9294
• Electronic ISSN: 1558-2531
• Publisher: Institute of Electrical and Electronics Engineers
• Peer Reviewed: Peer Reviewed
• Volume: 60
• Issue: 5
• Pages: 1209-1216
• DOI: https://doi.org/10.1109/TBME.2012.2228642
• Keywords: paramagnetic particles, magnetometer, magneto immunoassay, intracellular protein, prostate specific antigen
• Public URL: https://uwe-repository.worktribe.com/output/932517
• Publisher URL: http://dx.doi.org/10.1109/TBME.2012.2228642