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Influence of betaine and arginine supplementation of reduced protein diets on fatty acid composition and gene expression in the muscle and subcutaneous adipose tissue of cross-bred pigs

Madeira, Marta S.; Rolo, Eva A.; Alfaia, Cristina M.; Pires, Virgínia R.; Luxton, Richard; Doran, Olena; Bessa, RJB; Bessa, Rui J.B.; Prates, JAM; Prates, José A.M.

Authors

Marta S. Madeira

Eva A. Rolo

Cristina M. Alfaia

Virgínia R. Pires

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor

RJB Bessa

Rui J.B. Bessa

JAM Prates

José A.M. Prates



Abstract

© The Authors 2016. The isolated or combined effects of betaine and arginine supplementation of reduced protein diets (RPD) on fat content, fatty acid composition and mRNA levels of genes controlling lipid metabolism in pig m. longissimus lumborum and subcutaneous adipose tissue (SAT) were assessed. The experiment was performed on forty intact male pigs (Duroc×Large White×Landrace cross-breed) with initial and final live weights of 60 and 93 kg, respectively. Pigs were randomly assigned to one of the following five diets (n 8): 16·0 % of crude protein (control), 13·0 % of crude protein (RPD), RPD supplemented with 0·33 % of betaine, RPD supplemented with 1·5 % of arginine and RPD supplemented with 0·33 % of betaine and 1·5 % of arginine. Data confirmed that RPD increase intramuscular fat (IMF) content and total fat content in SAT. The increased total fat content in SAT was accompanied by higher GLUT type 4, lipoprotein lipase and stearoyl-CoA desaturase mRNA expression levels. In addition, the supplementation of RPD with betaine and/or arginine did not affect either IMF or total fat in SAT. However, dietary betaine supplementation slightly affected fatty acid composition in both muscle and SAT. This effect was associated with an increase of carnitine O-acetyltransferase mRNA levels in SAT but not in muscle, which suggests that betaine might be involved in the differential regulation of some key genes of lipid metabolism in pig muscle and SAT. Although the arginine-supplemented diet decreased the mRNA expression level of PPARG in muscle and SAT, it did not influence fat content or fatty acid composition in any of these pig tissues.

Journal Article Type Article
Publication Date Jan 26, 2016
Journal British Journal of Nutrition
Print ISSN 0007-1145
Electronic ISSN 1475-2662
Publisher Cambridge University Press (CUP)
Peer Reviewed Peer Reviewed
Volume 115
Issue 6
Pages 937-950
APA6 Citation Prates, J. A., Bessa, R. J., Madeira, M. S., Rolo, E. A., Alfaia, C. M., Pires, V. R., …Prates, J. (2016). Influence of betaine and arginine supplementation of reduced protein diets on fatty acid composition and gene expression in the muscle and subcutaneous adipose tissue of cross-bred pigs. British Journal of Nutrition, 115(6), 937-950. https://doi.org/10.1017/S0007114515005218
DOI https://doi.org/10.1017/S0007114515005218
Keywords betaine, arginine, reduced protein diets, lipid metabolism, intramuscular fat, fatty acid composition, pigs
Publisher URL http://dx.doi.org/10.1017/S0007114515005218
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