Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers

Jolly, Pawan; Estrela, Pedro; Ladomery, Michael

Abstract

© 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society. There are an increasing number of applications that have been developed for oligonucleotide-based biosensing systems in genetics and biomedicine. Oligonucleotidebased biosensors are those where the probe to capture the analyte is a strand of deoxyribonucleic acid (DNA), ribonucleic acid (RNA) or a synthetic analogue of naturally occurring nucleic acids. This review will shed light on various types of nucleic acids such as DNA and RNA (particularly microRNAs), their role and their application in biosensing. It will also cover DNA/RNA aptamers, which can be used as bioreceptors for a wide range of targets such as proteins, small molecules, bacteria and even cells. It will also highlight how the invention of synthetic oligonucleotides such as peptide nucleic acid (PNA) or locked nucleic acid (LNA) has pushed the limits of molecular biology and biosensor development to new perspectives. These technologies are very promising albeit still in need of development in order to bridge the gap between the laboratory-based status and the reality of biomedical applications.

Citation

Jolly, P., Estrela, P., & Ladomery, M. (2016). Oligonucleotide-based systems: DNA, microRNAs, DNA/RNA aptamers. Essays in Biochemistry, 60(1), 27-35. https://doi.org/10.1042/EBC20150004

• Journal Article Type: Article
• Acceptance Date: Dec 8, 2015
• Publication Date: Jun 30, 2016
• Deposit Date: Jul 12, 2016
• Publicly Available Date: Jun 30, 2017
• Journal: Essays in Biochemistry
• Print ISSN: 0071-1365
• Publisher: Portland Press
• Peer Reviewed: Peer Reviewed
• Volume: 60
• Issue: 1
• Pages: 27-35
• DOI: https://doi.org/10.1042/EBC20150004
• Keywords: oligonucleotides, microRNAs, aptamers, peptide nucleic acids, locked nucleic acids, biosensing
• Public URL: https://uwe-repository.worktribe.com/output/920282
• Publisher URL: http://dx.doi.org/10.1042/EBC20150004