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The determination of uric acid in human saliva by liquid chromatography with electrochemical detection

Honeychurch, Kevin C.

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Abstract

A method employing high-performance liquid chromatography with electrochemical detection (LC-ED) has been successfully developed for the determination of uric acid in human salvia. The optimal chromatographic conditions were found to comprise a mobile phase containing 5 % methanol, 95 % 50 mM trichloroacetic acid, adjusted to pH 2.7, in conjunction with a Hypersil C18 250 mm x 4.6 mm column at a flow rate of 0.6 mL min-1. Hydrodynamic voltammetric studies were undertaken to optimize the operating potentials required for electrochemical detection. It was found that an applied potential of +1.2 V was optimum for the determination of uric acid. The proposed method was evaluated by carrying out replicate uric acid determinations on spiked and unspiked human saliva samples (%CV = 8.8 %). The data suggest that the method holds promise for clinical applications.

Citation

Honeychurch, K. C. (2017). The determination of uric acid in human saliva by liquid chromatography with electrochemical detection

Journal Article Type Article
Acceptance Date Jan 16, 2017
Publication Date Jan 24, 2017
Deposit Date Feb 20, 2017
Publicly Available Date Mar 29, 2024
Journal Journal of Analytical, Bioanalytical and Separation Techniques
Peer Reviewed Peer Reviewed
Volume 2
Issue 1
Pages 1-5
Keywords uric acid, LC-ECD, human saliva, oxidative stress
Public URL https://uwe-repository.worktribe.com/output/899675
Publisher URL http://www.ommegaonline.org/article-details/The-Determination-of-Uric-Acid-in-Human-Saliva-by-Liquid-Chromatography-with-Electrochemical-Detection/1280

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