Dr Kevin Honeychurch Kevin.Honeychurch@uwe.ac.uk
Senior Lecturer in Forensic Science
The determination of uric acid in human saliva by liquid chromatography with electrochemical detection
Honeychurch, Kevin C.
Authors
Abstract
A method employing high-performance liquid chromatography with electrochemical detection (LC-ED) has been successfully developed for the determination of uric acid in human salvia. The optimal chromatographic conditions were found to comprise a mobile phase containing 5 % methanol, 95 % 50 mM trichloroacetic acid, adjusted to pH 2.7, in conjunction with a Hypersil C18 250 mm x 4.6 mm column at a flow rate of 0.6 mL min-1. Hydrodynamic voltammetric studies were undertaken to optimize the operating potentials required for electrochemical detection. It was found that an applied potential of +1.2 V was optimum for the determination of uric acid. The proposed method was evaluated by carrying out replicate uric acid determinations on spiked and unspiked human saliva samples (%CV = 8.8 %). The data suggest that the method holds promise for clinical applications.
Citation
Honeychurch, K. C. (2017). The determination of uric acid in human saliva by liquid chromatography with electrochemical detection
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 16, 2017 |
Publication Date | Jan 24, 2017 |
Deposit Date | Feb 20, 2017 |
Publicly Available Date | Mar 29, 2024 |
Journal | Journal of Analytical, Bioanalytical and Separation Techniques |
Peer Reviewed | Peer Reviewed |
Volume | 2 |
Issue | 1 |
Pages | 1-5 |
Keywords | uric acid, LC-ECD, human saliva, oxidative stress |
Public URL | https://uwe-repository.worktribe.com/output/899675 |
Publisher URL | http://www.ommegaonline.org/article-details/The-Determination-of-Uric-Acid-in-Human-Saliva-by-Liquid-Chromatography-with-Electrochemical-Detection/1280 |
Files
JABST-16-RA-1280 (3) Uric acid.pdf
(697 Kb)
PDF
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