Multispectral imaging for presymptomatic analysis of light leaf spot in oilseed rape

Veys, Charles; Chatziavgerinos, Fokion; AlSuwaidi, Ali; Hibbert, James; Hansen, Mark; Bernotas, Gytis; Smith, Melvyn; Yin, Hujun; Rolfe, Stephen; Grieve, Bruce

Abstract

Background: The use of spectral imaging within the plant phenotyping and breeding community has been increasing due its utility as a non-invasive diagnostic tool. However, there is a lack of imaging systems targeted specifically at plant science duties, resulting in low precision for canopy-scale measurements. This study trials a prototype multispectral system designed specifically for plant studies and looks at its use as an early detection system for visually asymptomatic disease phases, in this case Pyrenopeziza brassicae in Brassica napus. The analysis takes advantage of machine learning in the form of feature selection and novelty detection to facilitate the classification. An initial study into recording the morphology of the samples is also included to allow for further improvement to the system performance. Results: The proposed method was able to detect light leaf spot infection with 92% accuracy when imaging entire oilseed rape plants from above, 12 days after inoculation and 13 days before the appearance of visible symptoms. False colour mapping of spectral vegetation indices was used to quantify disease severity and its distribution within the plant canopy. In addition, the structure of the plant was recorded using photometric stereo, with the output influencing regions used for diagnosis. The shape of the plants was also recorded using photometric stereo, which allowed for reconstruction of the leaf angle and surface texture, although further work is needed to improve the fidelity due to uneven lighting distributions, to allow for reflectance compensation. Conclusions: The ability of active multispectral imaging has been demonstrated along with the improvement in time taken to detect light leaf spot at a high accuracy. The importance of capturing structural information is outlined, with its effect on reflectance and thus classification illustrated. The system could be used in plant breeding to enhance the selection of resistant cultivars, with its early and quantitative capability.

Citation

Veys, C., Chatziavgerinos, F., AlSuwaidi, A., Hibbert, J., Hansen, M., Bernotas, G., …Grieve, B. (2019). Multispectral imaging for presymptomatic analysis of light leaf spot in oilseed rape. Plant Methods, 15, Article 4. https://doi.org/10.1186/s13007-019-0389-9

• Journal Article Type: Article
• Acceptance Date: Jan 17, 2019
• Online Publication Date: Jan 24, 2019
• Publication Date: Jan 24, 2019
• Deposit Date: Jan 17, 2019
• Publicly Available Date: Jan 17, 2019
• Journal: Plant Methods
• Electronic ISSN: 1746-4811
• Publisher: BioMed Central
• Peer Reviewed: Peer Reviewed
• Volume: 15
• Article Number: 4
• DOI: https://doi.org/10.1186/s13007-019-0389-9
• Keywords: disease detection, light leaf spot, oilseed rape, multispectral, preprocessing, machine learning, support vector machine, novelty detection, orientation effects, photometric stereo
• Public URL: https://uwe-repository.worktribe.com/output/855008
• Publisher URL: https://doi.org/10.1186/s13007-019-0389-9