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Extraction-free, direct determination of caffeine in microliter volumes of beverages by thermal desorption-gas chromatography mass spectrometry

Peng, Xianglu; Brown, Melanie; Bowdler, Paul; Honeychurch, Kevin C.

Authors

Xianglu Peng

Melanie Brown

Paul Bowdler



Abstract

An extraction-free method requiring microliter (μL) volumes has been developed for the determination of caffeine in beverages.
Using a pyrolysis-gas chromatography mass spectrometry system, the conditions required for the direct thermal desorption-gas
chromatography mass spectrometry (TD-GC/MS) determination of caffeine were optimised. A 5 μL aliquot was introduced to the
thermal desorption unit, dried, and thermally desorbed to the GC/MS. *e response was linear over the range 10 to 500 μg/mL
(R2 = 0.996). *e theoretical limit of detection (3 σ) was 0.456 μg/mL. No interferences were recorded from endogenous beverage
components or from commonly occurring drugs, such as nicotine, ibuprofen, and paracetamol. Replicate caffeine determinations
on fortified latte style white coffee and Pepsi Max® gave mean recoveries of 93.4% (%CV = 4.1%) and 95.0% (%CV = 0.98%),
respectively. Good agreement was also obtained with the stated values of caffeine for an energy drink and for Coca-Cola®. *ese
data suggest that the method holds promise for the determination of caffeine in such samples.

Journal Article Type Article
Publication Date Apr 1, 2020
Journal International Journal of Analytical Chemistry
Print ISSN 1687-8760
Electronic ISSN 1687-8779
Publisher Hindawi
Peer Reviewed Peer Reviewed
Volume 2020
Article Number 5405184
Pages 1-7
APA6 Citation Peng, X., Brown, M., Bowdler, P., & Honeychurch, K. C. (2020). Extraction-free, direct determination of caffeine in microliter volumes of beverages by thermal desorption-gas chromatography mass spectrometry. International Journal of Analytical Chemistry, 2020, 1-7. https://doi.org/10.1155/2020/5405184
DOI https://doi.org/10.1155/2020/5405184
Keywords Analytical Chemistry

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Licence
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Copyright Statement
Copyright© 2020 Xianglu Peng et al. *is is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.





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