Extraction-free, direct determination of caffeine in microliter volumes of beverages by thermal desorption-gas chromatography mass spectrometry
Peng, Xianglu; Brown, Melanie; Bowdler, Paul; Honeychurch, Kevin C.
Kevin Honeychurch Kevin.Honeychurch@uwe.ac.uk
Senior Lecturer in Forensic Chemistry
An extraction-free method requiring microliter (μL) volumes has been developed for the determination of caffeine in beverages.
Using a pyrolysis-gas chromatography mass spectrometry system, the conditions required for the direct thermal desorption-gas
chromatography mass spectrometry (TD-GC/MS) determination of caffeine were optimised. A 5 μL aliquot was introduced to the
thermal desorption unit, dried, and thermally desorbed to the GC/MS. *e response was linear over the range 10 to 500 μg/mL
(R2 = 0.996). *e theoretical limit of detection (3 σ) was 0.456 μg/mL. No interferences were recorded from endogenous beverage
components or from commonly occurring drugs, such as nicotine, ibuprofen, and paracetamol. Replicate caffeine determinations
on fortified latte style white coffee and Pepsi Max® gave mean recoveries of 93.4% (%CV = 4.1%) and 95.0% (%CV = 0.98%),
respectively. Good agreement was also obtained with the stated values of caffeine for an energy drink and for Coca-Cola®. *ese
data suggest that the method holds promise for the determination of caffeine in such samples.
Peng, X., Brown, M., Bowdler, P., & Honeychurch, K. C. (2020). Extraction-free, direct determination of caffeine in microliter volumes of beverages by thermal desorption-gas chromatography mass spectrometry. International Journal of Analytical Chemistry, 2020, 1-7. https://doi.org/10.1155/2020/5405184
|Journal Article Type||Article|
|Acceptance Date||Mar 2, 2020|
|Online Publication Date||Apr 1, 2020|
|Publication Date||Apr 1, 2020|
|Deposit Date||Apr 2, 2020|
|Publicly Available Date||Apr 2, 2020|
|Journal||International Journal of Analytical Chemistry|
|Peer Reviewed||Peer Reviewed|
Caffeiene By TD CGMC International Journal Of Analytical Chemistry 2020 5405184
Publisher Licence URL
Copyright© 2020 Xianglu Peng et al. *is is an open access article distributed under the Creative Commons Attribution License,which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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