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Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene
Authors
P Ruddock
David J. Bird
A Hewer
A Van Schanke
D.H. Phillips
L.D. Peters
Abstract
Juvenile turbot (Scophthalmus maximus) were injected intraperitonteally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled 1 and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/108 nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P
Citation
Telli-Karakoç, F., Ruddock, P., Bird, D. J., Hewer, A., Van Schanke, A., Phillips, D., & Peters, L. (2002). Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene. Marine Environmental Research, 54(3-5), 511-515. https://doi.org/10.1016/S0141-1136%252802%252900192-7
| Journal Article Type | Article |
|---|---|
| Publication Date | Jan 1, 2002 |
| Journal | Marine Environmental Research |
| Print ISSN | 0141-1136 |
| Publisher | Elsevier |
| Peer Reviewed | Peer Reviewed |
| Volume | 54 |
| Issue | 3-5 |
| Pages | 511-515 |
| DOI | https://doi.org/10.1016/S0141-1136%252802%252900192-7 |
| Keywords | 7-Ethoxyresorufin O-deethylase activity, benzo[a]pyrene, bile, metabolites, DNA adduct, postlabelling |
| Publisher URL | http://dx.doi.org/10.1016/S0141-1136%2802%2900192-7 |