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Functional three-dimensional HepG2 aggregate cultures generated from an ultrasound trap: Comparison with HepG2 spheroids

Edwards, Gareth O.; Liu, Jian; Kuznetsova, Larisa A.; Xu, Jinsheng; Ma, Mingwen; Purcell, Wendy M.; Jackson, Simon K.; Coakley, W. Terence

Authors

Gareth O. Edwards

Jian Liu

Larisa A. Kuznetsova

Jinsheng Xu

Mingwen Ma

Wendy M. Purcell

Simon K. Jackson

W. Terence Coakley



Abstract

Three-dimensional culture systems are an ideal in vitro model being capable of sustaining cell functionalities in a manner that resembles the in vivo conditions. In the present study, we developed an ultrasound trap-based technique to rapidly produce HepC2 hepatocarcinomacell aggregates within 30 min. Enhanced junctional F-actin was observed at the points of cell-cell contact throughout the aggregates. HepC2 aggregates prepared by the ultrasound trap can be maintained in culture on a P-HEMA-coated surface for up to 3 weeks. The cells in these aggregates proliferated during the initial 3 days and cell number was consistent during the following maintenance period. Albumin secretion from these HepG2 aggregates recovered after 3 days of aggregate formation and remained relatively stable for the following 12 days. Cytochrome P450-1A1 activity was significantly enhanced after 6 days with maximal enzyme activity observed between 9 and 18 days. In addition, comparison experiments demonstrated that HepG2 aggregates generated by the ultrasound trap had comparable functional characterizations with HepG2 spheroids formed by a traditional gyrotatory-mediated method. Our results suggest that HepG2 aggregate cultures prepared through the ultrasound trap-based technique may provide a novel approach to produce in vitro models for hepatocyte functional studies. © 2007 Wiley-Liss, Inc.

Citation

Edwards, G. O., Liu, J., Kuznetsova, L. A., Xu, J., Ma, M., Purcell, W. M., …Coakley, W. T. (2007). Functional three-dimensional HepG2 aggregate cultures generated from an ultrasound trap: Comparison with HepG2 spheroids. Journal of Cellular Biochemistry, 102(5), 1180-1189. https://doi.org/10.1002/jcb.21345

Journal Article Type Article
Publication Date Dec 1, 2007
Journal Journal of Cellular Biochemistry
Print ISSN 0730-2312
Electronic ISSN 1097-4644
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 102
Issue 5
Pages 1180-1189
DOI https://doi.org/10.1002/jcb.21345
Keywords 3D culture, USWT, HepG2, F-actin, proliferation, albumin, P450-1A1
Public URL https://uwe-repository.worktribe.com/output/1033491
Publisher URL http://dx.doi.org/10.1002/jcb.21345


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