Rebecca A. Cue
Breed-associated variations in the sequence of the pig 3β- hydroxysteroid dehydrogenase gene
Cue, Rebecca A.; Nicolau-Solano, Silvia; McGivan, John D; Wood, Jeff D; Doran, O.
Authors
Silvia Nicolau-Solano
John D McGivan
Jeff D Wood
Olena Doran Olena.Doran@uwe.ac.uk
College Dean of Research and Enterprise
Abstract
The entire sequence of the pig 3β-hydroxysteroid dehydrogenase (3β-HSD) gene has recently become known. This gene is deemed to be important in androstenone metabolism in pig liver, and its defective expression has been shown to be related to androstenone accumulation in adipose tissue and the development of boar taint. The aim of the present work was to do the following: 1) define the structure of the pig 3β-HSD gene and 2) compare 3β-HSD DNA sequences from pigs of different breeds, which vary in adipose tissue androstenone levels, with the purpose of identifying a polymorphism that might be responsible for differential 3β-HSD expression. The 5′ flanking and the coding region of 3β-HSD were cloned and sequenced by conventional techniques. The 3β-HSD coding regions were identical in pigs of different breeds and in animals with high and low androstenone levels. Significant sequence variations were found in the 5′ flanking region of the 3β-HSD gene, where differences in the number of TTAT repeats and 3 SNP were observed. The SNP were associated with the number of the TTAT repeats. These variations in the DNA sequence of the 3β-HSD gene were not associated with the androstenone level in s.c. adipose tissue but were breed-dependent. The results of this work might be used for detection of the presence of Meishan genes in Western pig breeds, especially if the phenotype is not clearly established. © 2007 American Society of Animal Science. All rights reserved.
Journal Article Type | Article |
---|---|
Publication Date | Mar 1, 2007 |
Journal | Journal of Animal Science |
Print ISSN | 0021-8812 |
Electronic ISSN | 1525-3163 |
Publisher | Oxford University Press (OUP) |
Peer Reviewed | Peer Reviewed |
Volume | 85 |
Issue | 3 |
Pages | 571-576 |
DOI | https://doi.org/10.2527/jas.2006-366 |
Keywords | food quality, 3β-hydroxysteroid dehydrogenase, gene, pig, polymorphism |
Public URL | https://uwe-repository.worktribe.com/output/1032355 |
Publisher URL | http://dx.doi.org/10.2527/jas.2006-366 |
Additional Information | Corporate Creators : University of Bristol |
You might also like
Downloadable Citations
About UWE Bristol Research Repository
Administrator e-mail: repository@uwe.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search