Development of an amperometric screen-printed galactose biosensor for serum analysis

Pemberton, Roy M.; Kanyong, Prosper; Pemberton, Roy; Jackson, Simon K.; Hart, John P.

Abstract

The development of a disposable amperometric biosensor for the measurement of circulating galactose in serum is described. The biosensor comprises a screen-printed carbon electrode (SPCE), incorporating the electrocatalyst cobalt phthalocyanine (CoPC), which is covered by a permselective cellulose acetate (CA) membrane and a layer of immobilized galactose oxidase (GALOX). The optimal response of the biosensor, designated as GALOX-CA-CoPC-SPCE, was obtained by systematically examining the effects of enzyme loading, temperature, pH, and buffer strength. The optimal performance of the biosensor occurred with 2 U of GALOX, at 35 °C, using 50 mM phosphate buffer solution (pH 7.0). The sensitivity was 7.00 μA mM-1 cm-2 and the linear range from 0.1 to 25 mM with a calculated limit of detection (LOD) of 0.02 mM; this concentration range and LOD are appropriate to diagnose galactosemia, i.e., concentrations >1.1 mM in infants. When the biosensor was used in conjunction with amperometry in stirred solution for the analysis of serum, the precision values obtained on unspiked (endogenous level of 0.153 mM) and spiked serum (1 mM added) (n = 6) were 1.10% and 0.11%, respectively, with a calculated recovery of 99.9%. © 2013 Elsevier Inc. All rights reserved.

Citation

Pemberton, R. M., Kanyong, P., Pemberton, R., Jackson, S. K., & Hart, J. P. (2013). Development of an amperometric screen-printed galactose biosensor for serum analysis. Analytical Biochemistry, 435(2), 114-119. https://doi.org/10.1016/j.ab.2013.01.006

• Journal Article Type: Article
• Publication Date: Feb 19, 2013
• Journal: Analytical Biochemistry
• Print ISSN: 0003-2697
• Electronic ISSN: 1096-0309
• Publisher: Elsevier Masson
• Peer Reviewed: Peer Reviewed
• Volume: 435
• Issue: 2
• Pages: 114-119
• DOI: https://doi.org/10.1016/j.ab.2013.01.006
• Keywords: galactose, galactosemia, galactose oxidase, hydrogen peroxide, cellulose acetate, amperometry
• Public URL: https://uwe-repository.worktribe.com/output/932775
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0003269713000134