@article { , title = {Substantial high-affinity methanotroph populations in Andisols effect high rates of atmospheric methane oxidation}, abstract = {Methanotrophic bacteria in soils derived from volcanic ash (Andisols) were characterized via time series 13C-phospholipid fatty acid (PLFA) labelling. Three Andisols were incubated under 2 ppmv 13CH 4 for up to 18 weeks, thus enabling high-affinity methanotrophs to be selectively characterized and quantified. PLFA profiles from all soils were broadly similar, but the magnitude of the high-affinity methanotrophic populations determined through 13C-PLFA-stable isotope probing displayed sizeable differences. Substantial incorporation of 13C indicated very large high-affinity methanotrophic populations in two of the soils. Such high values are far in excess (10×) of those observed for a range of mineral soils incubated under similar conditions (Bull et al., 2000; Maxfield et al., 2006; 2008a, b). Two of the three Andisols studied also displayed high but variable CH 4 oxidation rates ranging from 0.03 to 1.58 nmolCH 4 g -1 d.wt. h -1. These findings suggest that Andisols, a previously unstudied soil class with respect to high-affinity methanotrophic bacteria, may oxidize significant amounts of atmospheric methane despite their low areal coverage globally. © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd.}, doi = {10.1111/j.1758-2229.2009.00071.x}, eissn = {1758-2229}, issue = {5}, journal = {Environmental Microbiology Reports}, pages = {450-456}, publicationstatus = {Published}, publisher = {Wiley}, url = {https://uwe-repository.worktribe.com/output/991957}, volume = {1}, keyword = {Centre for Research in Biosciences, Andisols, atmospheric methane oxidation, methanotroph populations}, year = {2009}, author = {Hornibrook, Ed R C and Maxfield, Pete J. and Evershed, Richard P.} }