@article { , title = {Relationship between vaginal microbial dysbiosis, inflammation, and pregnancy outcomes in cervical cerclage}, abstract = {Preterm birth, the leading cause of death in children under 5 years, may be caused by inflammation triggered by ascending vaginal infection. About 2 million cervical cerclages are performed annually to prevent preterm birth. The procedure is thought to provide structural support and maintain the endocervical mucus plug as a barrier to ascending infection. Two types of suture material are used for cerclage: monofilament or multifilament braided. Braided sutures are most frequently used, although no evidence exists to favor them over monofilament sutures. We assessed birth outcomes in a retrospective cohort of 678 women receiving cervical cerclage in five UK university hospitals and showed that braided cerclage was associated with increased intrauterine death (15\% versus 5\%; P = 0.0001) and preterm birth (28\% versus 17\%; P = 0.0006) compared to monofilament suture. To understand the potential underlying mechanism, we performed a prospective, longitudinal study of the vaginal microbiome in women at risk of preterm birth because of short cervical length (≥25 mm) who received braided (n = 25) or monofilament (n = 24) cerclage under comparable circumstances. Braided suture induced a persistent shift toward vaginal microbiome dysbiosis characterized by reduced Lactobacillus spp. and enrichment of pathobionts. Vaginal dysbiosis was associated with inflammatory cytokine and interstitial collagenase excretion into cervicovaginal fluid and premature cervical remodeling. Monofilament suture had comparatively minimal impact upon the vaginal microbiome and its interactions with the host. These data provide in vivo evidence that a dynamic shift of the human vaginal microbiome toward dysbiosis correlates with preterm birth.}, doi = {10.1126/scitranslmed.aag1026}, eissn = {1946-6242}, issn = {1946-6234}, issue = {350}, journal = {Science Translational Medicine}, publicationstatus = {Published}, publisher = {American Association for the Advancement of Science}, url = {https://uwe-repository.worktribe.com/output/3453599}, volume = {8}, year = {2016}, author = {McDonald, Julie A.K. and Kindinger, Lindsay M. and MacIntyre, David A. and Lee, Yun S. and Marchesi, Julian R. and Smith, Ann and Terzidou, Vasso and Cook, Joanna R. and Lees, Christoph and Israfil-Bayli, Fidan and Faiza, Yazmin and Toozs-Hobson, Philip and Slack, Mark and Cacciatore, Stefano and Holmes, Elaine and Nicholson, Jeremy K. and Teoh, T. G. and Bennett, Phillip R.} }